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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
30/09/2020 |
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Data da última atualização: |
07/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENGISTU, F. G.; MOTOIKE, S. Y.; CAIXETA, E. T.; CRUZ, C. D. |
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Afiliação: |
Fekadu Gebretensay Mengistu, Universidade Federal de Viçosa, Departamento de Fitotecnia; Sérgio Yoshimitsu Motoike, Universidade Federal de Viçosa, Departamento de Fitotecnia; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa; Cosme Damião Cruz, Universidade Federal de Viçosa, Departamento de Biologia Geral. |
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Título: |
Cross-species amplification and characterization of new microsatellite markers for the macaw palm, Acrocomia aculeata (Arecaceae). |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plant Genetic Resources: Characterization and Utilization, v. 14 , n. 3 , p. , 2016. |
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DOI: |
https://doi.org/10.1017/S1479262115000179 |
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Idioma: |
Inglês |
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Conteúdo: |
Microsatellites or simple sequence repeats (SSRs) are useful molecular markers allowing for efficient conservation and sustainable use of genetic resources of plant species. Development of SSR marker system for new species is a very expensive task and time consuming. Crossspecies amplification of microsatellite loci is considered as a cost-effective approach for developing microsatellite markers for new species. The aim of this work was to examine the transferability of some SSR markers of two Arecaceae species (Astrocaryum aculeatum and Elaeis oleifera), in Acrocomia aculeata. Out of the total markers analysed 44% of the markers successfully amplified the genomic DNA in A. aculeata, of which 26% were polymorphic detecting a range of three to eight alleles with an average of 4.5 per locus. High average percentage of polymorphic loci (P ¼ 71.2%) per provenance was obtained within a range of 57?100% detecting genetic variation in A. aculeata germplasm collections. The polymorphic markers detected a positive inbreeding coefficient (F . 0) per locus revealing heterozygote deficiency in the accessions that were analysed. As the cross-amplification was at family level, in which the taxonomic distance is relatively wider between the sources (A. aculeatum and E. oleifera) and the target (A. aculeata) species, the amplification success was relatively low. However, the results are promising and implicated that high cross-amplification success could be achieved at species or genus level in A. aculeata. The markers will contribute towards the domestication of the potential macaw palm through realizing various studies such as population genetics, germplasm characterization, genetic improvement and conservation. MenosMicrosatellites or simple sequence repeats (SSRs) are useful molecular markers allowing for efficient conservation and sustainable use of genetic resources of plant species. Development of SSR marker system for new species is a very expensive task and time consuming. Crossspecies amplification of microsatellite loci is considered as a cost-effective approach for developing microsatellite markers for new species. The aim of this work was to examine the transferability of some SSR markers of two Arecaceae species (Astrocaryum aculeatum and Elaeis oleifera), in Acrocomia aculeata. Out of the total markers analysed 44% of the markers successfully amplified the genomic DNA in A. aculeata, of which 26% were polymorphic detecting a range of three to eight alleles with an average of 4.5 per locus. High average percentage of polymorphic loci (P ¼ 71.2%) per provenance was obtained within a range of 57?100% detecting genetic variation in A. aculeata germplasm collections. The polymorphic markers detected a positive inbreeding coefficient (F . 0) per locus revealing heterozygote deficiency in the accessions that were analysed. As the cross-amplification was at family level, in which the taxonomic distance is relatively wider between the sources (A. aculeatum and E. oleifera) and the target (A. aculeata) species, the amplification success was relatively low. However, the results are promising and implicated that high cross-amplification success could be achieved at species or genus leve... Mostrar Tudo |
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Palavras-Chave: |
SSRs. |
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Thesagro: |
Macaúba. |
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Thesaurus Nal: |
Biodiesel; Domestication; Null alleles. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216307/1/Mengistu-et-al.-2015-1.pdf
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Marc: |
LEADER 02475naa a2200229 a 4500
001 2125189
005 2020-10-07
008 2016 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S1479262115000179$2DOI
100 1 $aMENGISTU, F. G.
245 $aCross-species amplification and characterization of new microsatellite markers for the macaw palm, Acrocomia aculeata (Arecaceae).$h[electronic resource]
260 $c2016
520 $aMicrosatellites or simple sequence repeats (SSRs) are useful molecular markers allowing for efficient conservation and sustainable use of genetic resources of plant species. Development of SSR marker system for new species is a very expensive task and time consuming. Crossspecies amplification of microsatellite loci is considered as a cost-effective approach for developing microsatellite markers for new species. The aim of this work was to examine the transferability of some SSR markers of two Arecaceae species (Astrocaryum aculeatum and Elaeis oleifera), in Acrocomia aculeata. Out of the total markers analysed 44% of the markers successfully amplified the genomic DNA in A. aculeata, of which 26% were polymorphic detecting a range of three to eight alleles with an average of 4.5 per locus. High average percentage of polymorphic loci (P ¼ 71.2%) per provenance was obtained within a range of 57?100% detecting genetic variation in A. aculeata germplasm collections. The polymorphic markers detected a positive inbreeding coefficient (F . 0) per locus revealing heterozygote deficiency in the accessions that were analysed. As the cross-amplification was at family level, in which the taxonomic distance is relatively wider between the sources (A. aculeatum and E. oleifera) and the target (A. aculeata) species, the amplification success was relatively low. However, the results are promising and implicated that high cross-amplification success could be achieved at species or genus level in A. aculeata. The markers will contribute towards the domestication of the potential macaw palm through realizing various studies such as population genetics, germplasm characterization, genetic improvement and conservation.
650 $aBiodiesel
650 $aDomestication
650 $aNull alleles
650 $aMacaúba
653 $aSSRs
700 1 $aMOTOIKE, S. Y.
700 1 $aCAIXETA, E. T.
700 1 $aCRUZ, C. D.
773 $tPlant Genetic Resources: Characterization and Utilization$gv. 14 , n. 3 , p. , 2016.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 5 | |
| 1. |  | NETTO, A. J.; GAMA, M. A. S. da; GUIDO, S. I.; INÁCIO, J. G.; CHAGAS, J. C. C.; RAMIN, M.; RABELO, M. N.; FÉLIX, S. B.; SILVA, C. S. DA; FERREIRA, M. DE A. Full-fat corn germ in diets for dairy cows as an alternative to ground corn. Journal of Dairy Research, v. 90, n. 2, p. 111-117, may 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 2. | | SILVA, A. P. F.; GAMA, M. A. S. da; SILVA, E. A. M.; RIBEIRO, E. F.; FELIX, S. B.; MONTEIRO C. C. F.; MORA-LUNA, R. E.; OLIVEIRA, J. C. V. DE; SANTOS, D. C. DOS; FERREIRA, M. A. DE. Full-fat corn germ improves the performance and milk fat yield of Girolando cows fed sugarcane bagasse and cactus cladodes as forage sources. Tropical Animal Health and Production, v. 56, art. 104, 2024. 9 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 3. | | GAMA, M. A. S. da; GOTTSCHALK, L. M. F.; WILHELM, A. E.; OLIVEIRA, A. H.; BORGES, C. A. V.; PAULA, T. A.; FÉLIX, S. B.; FERREIRA, M. A.; ANTONIASSI, R. Total phenolic content in milk from cows fed cactus (Opuntia stricta) cladodes as a partial substitute for sorghum silage in soybean oil-supplemented diets. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 28., 2022. Anais... [Online]: SBCTA; Embrapa, 2022. Evento online. 5 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 4. | | GAMA, M. A. S. da; GOTTSCHALK, L. M. F.; WILHELM, A. E.; OLIVEIRA, A. H.; BORGES, C. A. V.; PAULA, T. A.; FÉLIX, S. B.; FERREIRA, M. A.; ANTONIASSI, R. Total phenolic content in milk from cows fed cactus (Opuntia stricta) cladodes as a partial substitute for sorghum silage in soybean oil-supplemented diets. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 28., 2022. Anais... Campinas: Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2022. Evento online.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | GAMA, M. A. S. da; GOTTSCHALK, L. M. F.; WILHELM, A. E.; OLIVEIRA, A. H.; BORGES, C. A. V.; PAULA, T. A.; FÉLIX, S. B.; FERREIRA, M. A.; ANTONIASSI, R. Total phenolic content in milk from cows fed cactus (Opuntia stricta) cladodes as a partial substitute for sorghum silage in soybean oil-supplemented diets. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 28., 2022. Anais... [Online]: SBCTA; Embrapa, 2022. Evento online. 5 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 5 | |
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